Journal: Sexual Medicine

Article Title: Effects of endoplasmic reticulum stress on erectile function in rats with cavernous nerve injury

doi: 10.1093/sexmed/qfad050

Figure Lengend Snippet: 4-Phenylbutyric acid increases α-SMA expression and decreases osteopontin expression. (A) Immunofluorescence staining of α-SMA and OPN in cavernous tissue (day 28). (B-D) Western blot analysis was employed to quantitate the levels of α-SMA and OPN. Data are reported as mean ± SD (n = 3 per group). * P < .05 vs the control group at 14 days per ANOVA and Bonferroni multiple-comparison test. * * P < .05 vs the control group at 28 days per ANOVA and Bonferroni multiple-comparison test. # P < .05 vs the experimental group at 14 days per a t -test. α-SMA, α–smooth muscle actin; ANOVA, analysis of variance.

Article Snippet: These membranes were blocked with 5% skimmed milk for 2 hours and incubated overnight at 4 °C with the following primary antibodies: anti–neuronal nitric oxide synthase (anti-nNOS, 1:1000; Abcam), anti-GRP78 (1:1000; Abcam), anti-CHOP (1:1000; CST), anti-PERK (1:1000; CST), anti–p-PERK (1:1000; CST), anti–caspase 3 (1:1000; Novus Biologicals), anti–α-SMA (1:1000; CST), and anti-OPN (1:1000; Novus Biologicals).

Techniques: Expressing, Immunofluorescence, Staining, Western Blot, Comparison